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monoclonal antibody m38  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank monoclonal antibody m38
    Co-cultures retain zone separation for up to 7 days (SZ: surface zone; DZ: deep zone) . Boxplot showing quantification of SV40 large T-antigen gene expression in the MLO-Y4/MC3T3-E1(14) co-culture after 7 days by relative RT-qPCR (A) expressed as REU and normalized to Gapdh expression. Significant differences obtained by GLM of log 10 data between surface and deep zones denoted by ** P < 0.01. Significant differences from pairwise comparisons, within each zone, between independent experiments denoted by “a” with respect to experiment 1 (three independent experiments, n = 3 for surface and 4 for deep zones). Fluorescent photomicrograph of transverse cryosection from day 7 MLO-Y4/MC3T3-E1(14) co-culture shows immunolabelling for SV40 large T-antigen (red) and cell nuclei stain (blue) in osteocytes only, represented by the purple color (red and blue co-localization) (B) . However, no SV40 large T-antigen immunostaining in the osteoblasts was present (three independent experiments, n = 3). SZ, surface zone; DZ, deep zone. Fluorescent photomicrograph of transverse cryosection from BMP-2 treated MLO-Y4/MG63 co-cultures at day 5 (C) revealed presence of type I pro-collagen in the upper layer of cells and in cells up to 100 μm beneath the surface, which are all MG63 cells since <t>M38</t> antibody does not recognize mouse type I pro-collagen (two independent experiments, n = 3).
    Monoclonal Antibody M38, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/type+i+collagen+antibody+m38/anti-collagen+type+I/pmc04260042-103-6-27
    Average 94 stars, based on 25 article reviews
    monoclonal antibody m38 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "A New Method to Investigate How Mechanical Loading of Osteocytes Controls Osteoblasts"

    Article Title: A New Method to Investigate How Mechanical Loading of Osteocytes Controls Osteoblasts

    Journal: Frontiers in Endocrinology

    doi: 10.3389/fendo.2014.00208

    Co-cultures retain zone separation for up to 7 days (SZ: surface zone; DZ: deep zone) . Boxplot showing quantification of SV40 large T-antigen gene expression in the MLO-Y4/MC3T3-E1(14) co-culture after 7 days by relative RT-qPCR (A) expressed as REU and normalized to Gapdh expression. Significant differences obtained by GLM of log 10 data between surface and deep zones denoted by ** P < 0.01. Significant differences from pairwise comparisons, within each zone, between independent experiments denoted by “a” with respect to experiment 1 (three independent experiments, n = 3 for surface and 4 for deep zones). Fluorescent photomicrograph of transverse cryosection from day 7 MLO-Y4/MC3T3-E1(14) co-culture shows immunolabelling for SV40 large T-antigen (red) and cell nuclei stain (blue) in osteocytes only, represented by the purple color (red and blue co-localization) (B) . However, no SV40 large T-antigen immunostaining in the osteoblasts was present (three independent experiments, n = 3). SZ, surface zone; DZ, deep zone. Fluorescent photomicrograph of transverse cryosection from BMP-2 treated MLO-Y4/MG63 co-cultures at day 5 (C) revealed presence of type I pro-collagen in the upper layer of cells and in cells up to 100 μm beneath the surface, which are all MG63 cells since M38 antibody does not recognize mouse type I pro-collagen (two independent experiments, n = 3).
    Figure Legend Snippet: Co-cultures retain zone separation for up to 7 days (SZ: surface zone; DZ: deep zone) . Boxplot showing quantification of SV40 large T-antigen gene expression in the MLO-Y4/MC3T3-E1(14) co-culture after 7 days by relative RT-qPCR (A) expressed as REU and normalized to Gapdh expression. Significant differences obtained by GLM of log 10 data between surface and deep zones denoted by ** P < 0.01. Significant differences from pairwise comparisons, within each zone, between independent experiments denoted by “a” with respect to experiment 1 (three independent experiments, n = 3 for surface and 4 for deep zones). Fluorescent photomicrograph of transverse cryosection from day 7 MLO-Y4/MC3T3-E1(14) co-culture shows immunolabelling for SV40 large T-antigen (red) and cell nuclei stain (blue) in osteocytes only, represented by the purple color (red and blue co-localization) (B) . However, no SV40 large T-antigen immunostaining in the osteoblasts was present (three independent experiments, n = 3). SZ, surface zone; DZ, deep zone. Fluorescent photomicrograph of transverse cryosection from BMP-2 treated MLO-Y4/MG63 co-cultures at day 5 (C) revealed presence of type I pro-collagen in the upper layer of cells and in cells up to 100 μm beneath the surface, which are all MG63 cells since M38 antibody does not recognize mouse type I pro-collagen (two independent experiments, n = 3).

    Techniques Used: Gene Expression, Co-Culture Assay, Quantitative RT-PCR, Expressing, Staining, Immunostaining

    Effects of BMP-2 treatment on type I pro-collagen synthesis in co-cultures of MLO-Y4 and MG63 cells (type I pro-collagen: red; DAPI: blue) . Confocal microscope image of the surface cells from an untreated 3D co-culture revealed presence of particulate type I pro-collagen in all surface cells (A) . Fluorescence microscope images of transverse cryosections from BMP-2 treated 3D co-cultures at day 5 (B) revealed presence of type I pro-collagen in the upper layer of cells and in cells up to 100 μm beneath the surface, which are all MG63 cells since M38 antibody does not recognize mouse type I pro-collagen.
    Figure Legend Snippet: Effects of BMP-2 treatment on type I pro-collagen synthesis in co-cultures of MLO-Y4 and MG63 cells (type I pro-collagen: red; DAPI: blue) . Confocal microscope image of the surface cells from an untreated 3D co-culture revealed presence of particulate type I pro-collagen in all surface cells (A) . Fluorescence microscope images of transverse cryosections from BMP-2 treated 3D co-cultures at day 5 (B) revealed presence of type I pro-collagen in the upper layer of cells and in cells up to 100 μm beneath the surface, which are all MG63 cells since M38 antibody does not recognize mouse type I pro-collagen.

    Techniques Used: Microscopy, Co-Culture Assay, Fluorescence

    Related Articles

    Binding Assay:

    Article Title: Chondrocyte Culture in Three Dimensional Alginate Sulfate Hydrogels Promotes Proliferation While Maintaining Expression of Chondrogenic Markers
    Article Snippet: L-ascorbic acid phosphate magnesium salt was obtained from Wako (IG instrumenten-Gesellschaft AG; Zurich). .. Type II collagen antibody II-II6B3, type I collagen antibody M38, proteoglycan, hyaluronic acid binding region (12/21/1-C-6), and AIIB2 beta1 integrin antibody was from Developmental Studies Hybridoma Bank, University of Iowa. ..

    Article Title: Chondrocyte Culture in Three Dimensional Alginate Sulfate Hydrogels Promotes Proliferation While Maintaining Expression of Chondrogenic Markers
    Article Snippet: L-ascorbic acid phosphate magnesium salt was obtained from Wako (IG instrumenten-Gesellschaft AG; Zurich). .. Type II collagen antibody II-II6B3, type I collagen antibody M38, proteoglycan, hyaluronic acid binding region (12/21/1-C-6), and AIIB2 beta1 integrin antibody was from Developmental Studies Hybridoma Bank, University of Iowa. ..



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    monoclonal antibody m38  (Developmental Studies Hybridoma Bank)
    94
    Developmental Studies Hybridoma Bank monoclonal antibody m38
    Co-cultures retain zone separation for up to 7 days (SZ: surface zone; DZ: deep zone) . Boxplot showing quantification of SV40 large T-antigen gene expression in the MLO-Y4/MC3T3-E1(14) co-culture after 7 days by relative RT-qPCR (A) expressed as REU and normalized to Gapdh expression. Significant differences obtained by GLM of log 10 data between surface and deep zones denoted by ** P < 0.01. Significant differences from pairwise comparisons, within each zone, between independent experiments denoted by “a” with respect to experiment 1 (three independent experiments, n = 3 for surface and 4 for deep zones). Fluorescent photomicrograph of transverse cryosection from day 7 MLO-Y4/MC3T3-E1(14) co-culture shows immunolabelling for SV40 large T-antigen (red) and cell nuclei stain (blue) in osteocytes only, represented by the purple color (red and blue co-localization) (B) . However, no SV40 large T-antigen immunostaining in the osteoblasts was present (three independent experiments, n = 3). SZ, surface zone; DZ, deep zone. Fluorescent photomicrograph of transverse cryosection from BMP-2 treated MLO-Y4/MG63 co-cultures at day 5 (C) revealed presence of type I pro-collagen in the upper layer of cells and in cells up to 100 μm beneath the surface, which are all MG63 cells since <t>M38</t> antibody does not recognize mouse type I pro-collagen (two independent experiments, n = 3).
    Monoclonal Antibody M38, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/type+i+collagen+antibody+m38/anti-collagen+type+I/pmc04260042-103-6-27
    Average 94 stars, based on 1 article reviews
    monoclonal antibody m38 - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    type i collagen antibody m38  (Developmental Studies Hybridoma Bank)
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    Developmental Studies Hybridoma Bank type i collagen antibody m38
    Co-cultures retain zone separation for up to 7 days (SZ: surface zone; DZ: deep zone) . Boxplot showing quantification of SV40 large T-antigen gene expression in the MLO-Y4/MC3T3-E1(14) co-culture after 7 days by relative RT-qPCR (A) expressed as REU and normalized to Gapdh expression. Significant differences obtained by GLM of log 10 data between surface and deep zones denoted by ** P < 0.01. Significant differences from pairwise comparisons, within each zone, between independent experiments denoted by “a” with respect to experiment 1 (three independent experiments, n = 3 for surface and 4 for deep zones). Fluorescent photomicrograph of transverse cryosection from day 7 MLO-Y4/MC3T3-E1(14) co-culture shows immunolabelling for SV40 large T-antigen (red) and cell nuclei stain (blue) in osteocytes only, represented by the purple color (red and blue co-localization) (B) . However, no SV40 large T-antigen immunostaining in the osteoblasts was present (three independent experiments, n = 3). SZ, surface zone; DZ, deep zone. Fluorescent photomicrograph of transverse cryosection from BMP-2 treated MLO-Y4/MG63 co-cultures at day 5 (C) revealed presence of type I pro-collagen in the upper layer of cells and in cells up to 100 μm beneath the surface, which are all MG63 cells since <t>M38</t> antibody does not recognize mouse type I pro-collagen (two independent experiments, n = 3).
    Type I Collagen Antibody M38, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/type+i+collagen+antibody+m38/anti-collagen+type+I/10__1089_slash_ten__tea__2013__0544-42-5-23
    Average 94 stars, based on 1 article reviews
    type i collagen antibody m38 - by Bioz Stars, 2026-09
    94/100 stars
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    Image Search Results


    Co-cultures retain zone separation for up to 7 days (SZ: surface zone; DZ: deep zone) . Boxplot showing quantification of SV40 large T-antigen gene expression in the MLO-Y4/MC3T3-E1(14) co-culture after 7 days by relative RT-qPCR (A) expressed as REU and normalized to Gapdh expression. Significant differences obtained by GLM of log 10 data between surface and deep zones denoted by ** P < 0.01. Significant differences from pairwise comparisons, within each zone, between independent experiments denoted by “a” with respect to experiment 1 (three independent experiments, n = 3 for surface and 4 for deep zones). Fluorescent photomicrograph of transverse cryosection from day 7 MLO-Y4/MC3T3-E1(14) co-culture shows immunolabelling for SV40 large T-antigen (red) and cell nuclei stain (blue) in osteocytes only, represented by the purple color (red and blue co-localization) (B) . However, no SV40 large T-antigen immunostaining in the osteoblasts was present (three independent experiments, n = 3). SZ, surface zone; DZ, deep zone. Fluorescent photomicrograph of transverse cryosection from BMP-2 treated MLO-Y4/MG63 co-cultures at day 5 (C) revealed presence of type I pro-collagen in the upper layer of cells and in cells up to 100 μm beneath the surface, which are all MG63 cells since M38 antibody does not recognize mouse type I pro-collagen (two independent experiments, n = 3).

    Journal: Frontiers in Endocrinology

    Article Title: A New Method to Investigate How Mechanical Loading of Osteocytes Controls Osteoblasts

    doi: 10.3389/fendo.2014.00208

    Figure Lengend Snippet: Co-cultures retain zone separation for up to 7 days (SZ: surface zone; DZ: deep zone) . Boxplot showing quantification of SV40 large T-antigen gene expression in the MLO-Y4/MC3T3-E1(14) co-culture after 7 days by relative RT-qPCR (A) expressed as REU and normalized to Gapdh expression. Significant differences obtained by GLM of log 10 data between surface and deep zones denoted by ** P < 0.01. Significant differences from pairwise comparisons, within each zone, between independent experiments denoted by “a” with respect to experiment 1 (three independent experiments, n = 3 for surface and 4 for deep zones). Fluorescent photomicrograph of transverse cryosection from day 7 MLO-Y4/MC3T3-E1(14) co-culture shows immunolabelling for SV40 large T-antigen (red) and cell nuclei stain (blue) in osteocytes only, represented by the purple color (red and blue co-localization) (B) . However, no SV40 large T-antigen immunostaining in the osteoblasts was present (three independent experiments, n = 3). SZ, surface zone; DZ, deep zone. Fluorescent photomicrograph of transverse cryosection from BMP-2 treated MLO-Y4/MG63 co-cultures at day 5 (C) revealed presence of type I pro-collagen in the upper layer of cells and in cells up to 100 μm beneath the surface, which are all MG63 cells since M38 antibody does not recognize mouse type I pro-collagen (two independent experiments, n = 3).

    Article Snippet: Type I pro-collagen was labeled using monoclonal antibody M38 recognizing the C-terminus of pro-collagen I in a wide range of species except mouse [5 μg/mL; ( ); Developmental Studies Hybridoma Bank], CX43 using monoclonal antibody CXN-6 (8 μg/mL; Sigma) and E11 with goat anti-mouse podoplanin (E11) primary antibody (2.5 μg/mL; R&D Systems).

    Techniques: Gene Expression, Co-Culture Assay, Quantitative RT-PCR, Expressing, Staining, Immunostaining

    Effects of BMP-2 treatment on type I pro-collagen synthesis in co-cultures of MLO-Y4 and MG63 cells (type I pro-collagen: red; DAPI: blue) . Confocal microscope image of the surface cells from an untreated 3D co-culture revealed presence of particulate type I pro-collagen in all surface cells (A) . Fluorescence microscope images of transverse cryosections from BMP-2 treated 3D co-cultures at day 5 (B) revealed presence of type I pro-collagen in the upper layer of cells and in cells up to 100 μm beneath the surface, which are all MG63 cells since M38 antibody does not recognize mouse type I pro-collagen.

    Journal: Frontiers in Endocrinology

    Article Title: A New Method to Investigate How Mechanical Loading of Osteocytes Controls Osteoblasts

    doi: 10.3389/fendo.2014.00208

    Figure Lengend Snippet: Effects of BMP-2 treatment on type I pro-collagen synthesis in co-cultures of MLO-Y4 and MG63 cells (type I pro-collagen: red; DAPI: blue) . Confocal microscope image of the surface cells from an untreated 3D co-culture revealed presence of particulate type I pro-collagen in all surface cells (A) . Fluorescence microscope images of transverse cryosections from BMP-2 treated 3D co-cultures at day 5 (B) revealed presence of type I pro-collagen in the upper layer of cells and in cells up to 100 μm beneath the surface, which are all MG63 cells since M38 antibody does not recognize mouse type I pro-collagen.

    Article Snippet: Type I pro-collagen was labeled using monoclonal antibody M38 recognizing the C-terminus of pro-collagen I in a wide range of species except mouse [5 μg/mL; ( ); Developmental Studies Hybridoma Bank], CX43 using monoclonal antibody CXN-6 (8 μg/mL; Sigma) and E11 with goat anti-mouse podoplanin (E11) primary antibody (2.5 μg/mL; R&D Systems).

    Techniques: Microscopy, Co-Culture Assay, Fluorescence